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KMID : 0985420230450020065
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Laboratory Medicine and Quality Assurance
2023 Volume.45 No. 2 p.65 ~ p.69
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Reliability of Acinetobacter baumannii Identification with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
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Kim Jung-Lim
Kim Young-Ah
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Abstract
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Background : The purpose of this study was to evaluate the reliability of identification with the strains of Acinetobacter baumannii and non-baumanni Acinetobacter spp, determined with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), compared to the molecular genetic method.
Methods : A total of 225 blood isolates of A. baumannii and non-baumanni Acinetobacter spp. were included in this study. Species identification was performed using MALDI-TOF MS, blaOXA-51 polymerase chain reaction (PCR), and RNA polymerase β-subunit (rpoB) PCR-sequencing.
Results : The MALDI-TOF MS results showed 100% category agreement with that of blaOXA-51 PCR, which clearly distinguished A. baumannii from non-baumanni Acinetobacter spp. The species-concordance rate of non-baumanni Acinetobacter spp. was 73.7% (14/19) on the basis of rpoB sequence results.
Conclusions : MALDI-TOF MS is very useful in distinguishing between A. baumannii and non-baumanni Acinetobacter spp. accurately, which is useful information on the choice of therapeutic agents. However, there was a limitation in correctly identifying non-baumanni Acinetobacter spp.
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KEYWORD
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Species identification, Matrix-assisted laser desorption ionization-time of flight mass spectrometry, Acinetobacter baumannii, Non-baumanni Acinetobacter spp
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